Based on univariate analysis, PPFT, age, prostatic specific antigen (PSA), the percentage of free-PSA (%fPSA), total prostate volume (TPV), PI-RADS score and suspicious digital rectal exam (DRE) demonstrated statistical significance between biopsy-negative patients and PCa patients as well as biopsy-negative patients and HGPCa patients based on univariate analysis. This evidence concerns the gene KLK3 and posterior cortical atrophy.