In this work we identified new miRNAs regulating GPC3. Among them, 5 (namely miR-203a-3p miR-4510, miR-548aa, miR-376b-3p and miR-548v) inhibited GPC3 expression in HCC cells and acted as tumor suppressors in liver by inhibiting HCC cell growth and proliferation. This evidence concerns the gene GPC3 and neoplasm.