To this end, protein expression of total AKT (tAKT), AKT phosphorylated at serine 473 (pAKT-473), and AKT phosphorylated at threonine 308 (pAKT-308) were examined in both AF1q-knockdown and AF1q-overexpressing CRC cells by Western blot. This evidence concerns the gene AKT1 and colorectal carcinoma.