In liver cancer cells, treatment with PuGNAc and GlcNAc enhanced YAP O-GlcNAcylation (Supplementary Fig. 4d), and overexpression of OGT resulted in a similar enhancement of YAP O-GlcNAcylation, while knockdown of OGT led to suppression of YAP O-GlcNAcylation (Supplementary Fig. 4e). Here, OGT is linked to liver cancer.