We also examined O-T241-YAP in the same tissue microarray that was used in Fig. 1a, and found that O-T241-YAP was significantly associated with the levels of total-YAP, global O-GlcNAcylation, CTGF and Ki67 (Supplementary Fig. 8e), further suggesting that global O-GlcNAcylation might reinforce YAP expression via O-GlcNAcylation of YAP at Thr241, and O-GlcNAcylation of YAP at Thr241 might be involved in the regulation of TEAD-dependent transcription and cell proliferation in liver cancer cells. This evidence concerns the gene YAP1 and liver cancer.