In liposarcoma cells, we found that site directed mutagenesis of CpG sites affected ANRIL and p14ARF promoter activity respectively, and although mutation of a CpG site does not infer that methylation of the CpG would have the same effect, it does clearly suggest that these CpG sequences are important determinants regulating ANRIL and p14ARF expression. The gene discussed is CDKN2B-AS1; the disease is liposarcoma.