Simultaneously, the phosphorylation level of tau at multiple AD-associated sites was significantly increased in htau mice, while silencing ANP32A attenuated tau phosphorylation at Ser231/262/404 and increased unphosphorylated tau level at Ser195/198/199/202 (tau-1) (Additional file 3: Figure S1b, c). This evidence concerns the gene MAPT and Alzheimer disease.