Immunoprecipitation with the nitrosocysteine antibody followed by Western blotting with the HSP90 antibody (Fig 6B) or the SERCA2 antibody (Fig 6C) also indicated that these proteins are S-nitrosylated in response to Ova/Sugen5416-dependent PAH in the RV. This evidence concerns the gene ATP2A2 and pulmonary arterial hypertension.