HK2 and neoplasm: We found that depletion of Smad3 in colorectal cancer cells reduced HIF-1α expression, which was consistent with another work from our team also exhibited that Smad3 contributed to tumor glycolysis, which is key regulator in tumor glycolysis (data not shown, unpublished), these results implied that miR-1 regulated tumor glycolysis not only by directly targeting HIF-1α but also by Smad3-mediated HIF-1α, HK2 and MCT4 expression.