The results showed that miR-365 ameliorated DEX-induced suppression of osteogenesis via a direct interaction between miR-365 and the 3′-UTR of HDAC4 mRNA in osteoblasts, suggesting that miR-365 may be considered a promising therapeutic agent to treat glucocorticoid-induced osteoporosis. The gene discussed is HDAC4; the disease is osteoporosis.