The subcellular localization of p-Smad2 and p-Smad3 suggested that RGC32 activates the Smad/Sip1 pathway and promotes translocation of p-Smad2 and p-Smad3 into nucleus, thus inducing EMT and enhancing the metastatic ability of CRC cells. The gene discussed is RGCC; the disease is colorectal carcinoma.