By 6 h, infection with the PA14 ΔflgK mutant showed significantly decreased levels (~ 5–10-fold) of tlr4, tlr5, nlrc4, casp-4, il-18, and il-1β cytokine gene expression compared with PA14 wild-type (P < 0.05; Figure 2A). The gene discussed is TLR4; the disease is infection.