TLR4 and infection: After 6 h of hCFs infection with wild-type PAO1 and PA14, significant levels of TLR4, TLR5, NLRC4, native form of caspase-4 and IL-1β and IL-18 mature-formed proteins were detected by western blot with quantification of protein densitometry ratios relative to endogenous β-actin (Figure 2B, Supplementary Table 2).