Real-time PCR demonstrated that WT mice with anti-GBM GN had a marked increase in mRNA levels of IL-1β, TNF-α, and MCP-1 in the diseased kidney (Fig. 2A–C), which was infiltrated by large numbers of CD3+ T cells and F4/80+ macrophages (Fig. 2D–E). This evidence concerns the gene CCL2 and glioblastoma.