The ER+ cell lines MCF-7 and T47D, the triple-negative breast cancer cell lines MDA-MB-231, Her2+, and SKBR3, and the endothelial cell line human umbilical vein endothelial cells (HUVEC) were treated with various concentrations of nobiletin, and cell proliferation was assessed by comparison with non-treated control cells (Figure 1A). Here, ESR1 is linked to triple-negative breast carcinoma.