In this study, we investigated whether radiation could enhance PD-L1 expression in tumor cells, which may provide an effective milieu for anti-PD-L1 binding to tumor cells, as well as its impact on tumor growth suppression and survival, using murine HCC syngeneic to C3H/HeN mice as a preclinical model for HCC. The gene discussed is CD274; the disease is hepatocellular carcinoma.