To investigate the function and mechanisms of KCTD11 in HCC, stably ectopic expression of KCTD11 (marked with a Flag tag) was performed in a high metastatic HCC cell line (HCCLM3), while stably knock-down was performed in a low metastatic HCC cell line (Huh7), by transfection assay with lentiviral vectors. Here, KCTD11 is linked to hepatocellular carcinoma.