Given the role of ARF GTPases in controlling Golgi complex and PTM MT organization around the inclusion and given the fact that CT813 recruits both GDP- and GTP-bound ARF1 to the inclusion membrane (Fig. 4E), we investigated the respective roles of each nucleotide-bound form during infection in cells expressing low levels of HA-tagged ARF1 WT, ARF1 Q71L (GTP locked), or ARF1 T48N (GDP locked) in WT Chlamydia-infected cells. Here, ARF1 is linked to infection.