A recent report used L2 immunofluorescence microscopy with selective plasma membrane permeabilization to confirm that L2 can indeed use this TMD during infection to span vesicular membranes with a lumenal N-terminal domain (residues 1–45) and residues immediately downstream of the TMD (residues 68–170) residing in the cytosol, which is consistent with, but not proof of, L2 adopting a type-I transmembrane protein topology [49]. Here, SGCG is linked to infection.