JAM1 also interacts with another PDZ-containing domain, Afadin which is localized at the ependymal zonulae adherens and TJs of the third ventricle and of the aqueduct of Sylvius and whose genetic deletion induces hydrocephalus with disappearance of ependyma in the mouse midbrain and obliteration of the third ventricle [31]. The gene discussed is AFDN; the disease is Hydrocephalus.