We assessed next the ability of mono-dispersed c.1380delA CDH1 SB.mhdgc-1 gastric cancer cells to self-assemble and form multicellular spheroids (MCS), both as a measure of their self-renewal ability and tumorigenicity as well as to investigate the possible impact the loss of CDH1 might have on self-aggregation and the ability to form cell-to-cell contact. The gene discussed is CDH1; the disease is gastric cancer.