To determine whether the binding affinity of fusion proteins to NSCLC cells was correlated with the EGFR and IGF-1R expression levels, the expression of EGFR and IGF-1R on NSCLC cells was detected by Western blot analysis (Figure 2C), and two-way analysis of variance (ANOVA) and Bonferroni post-tests were carried out among the MFIs of A549, H460 and H520 cells (Prism 5 software). This evidence concerns the gene EGFR and non-small cell lung carcinoma.