In summary, this study firstly showed that the novel ligand-based EGFR/IGF-1R bispecific fusion protein EGF-IGF-LDP-AE not only demonstrated potent cytotoxicity to NSCLC cells in vitro, but also was highly effective in inhibiting the growth of A549 xenografts in vivo. Here, IGF1R is linked to non-small cell lung carcinoma.