To investigate the antitumor activity induced by AdC7-SP/E1A-ΔE3 via intravenous injection, we inoculate the mixture of Huh7 cells with matrigel under the skins in the xenograft mouse tumor models; when tumors reached 100–150 mm3, mice were intravenously injected with 1 × 109 PFU of AdC7-ΔE1A-ΔE3 or AdC7-SP/E1A-ΔE3. The gene discussed is DHTKD1; the disease is neoplasm.