We inoculated matrigel mixtures of NCI-H508 or Huh7 tumor cells under the skin; when the tumor volume reached 100-150 mm3, we treated them with PBS, AdC7-ΔE1A-ΔE3, AdC7-ΔE3, or AdC7-SP/E1A-ΔE3 through intratumoral injection. Here, DHTKD1 is linked to neoplasm.