Among these three genes, CACNA2D2 showed the best correlation with CDC42 as well as the best improvement on the prognostic value when combined with CDC42, so we took this gene to perform further validation of its clinical relevance in a third CRC cohort as well as to further perform in vitro and in vivo studies its relationship with CDC42. This evidence concerns the gene CACNA2D2 and colorectal carcinoma.