RCC2 and familial pancreatic carcinoma: Therefore we examined whether miR-1247 is able to interact directly with the 3′UTR of RCC2. The miR-1247 binding sequence at the 3′UTR of RCC2 mRNA (wt) was cloned downstream of the firefly luciferase reporter gene, and then cotransfected with mimic miR-1247 or AllStar negative control into the pancreatic cancer cell lines.