SCRIB and hepatocellular carcinoma: To further investigate whether Scrib translocates to the nucleus in actively proliferating cells, we prepared membrane, cytoplasmic and nuclear fractions from Huh-7 and Hepa1-6 HCC cells and detected Scrib in all 3 fractions (Figure 3E–3F), suggesting that Scrib not only translocated to the cytoplasm but also to the nucleus in actively proliferating cells.