Based on our aforementioned studies in patient tumor samples showing high levels of NFκB and XIAP in tumor embolic structures, we wanted to test if targeting the XIAP-NFκB anti-apoptotic signaling pathway can inhibit formation of tumor cell clusters and/or induce cell death using the high content IBC 3D spheroid assay developed herein. This evidence concerns the gene NFKB1 and neoplasm.