In this proof-of-concept study, a LEAPS vaccine for HER-2/neu-positive breast cancer was prepared by covalent attachment of the J-ICBL using a triglycine spacer to a prominent CTL-eliciting minimal CD8 T cell epitope from HER-2/neu (aa66-74: TYVPANASL) for BALB/c mice (J-HER) [24]. The gene discussed is CD8A; the disease is breast cancer.