This approach was named as SERPA and successfully applied to identify and character many kinds of autoantigens in various cancers, such as eukaryotic elongation factor 2 (EEF2), enolase1(ENO1), aldolase A (ALDOA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and heterogeneous nuclear ribonucleoproteins (HNRNP A2B1) in Melanoma [24], peroxiredoxin 6 in esophageal cancer, triophosphatase isomerase (Tim) [25], superoxide dismutase (MnSOD) in lung cancer [26], RS/DJ-1 in breast cancer [27]. The gene discussed is ENO1; the disease is breast carcinoma.