We do not know whether CM from ECs from lung, colon mucosa, and kidney increased NANOG protein levels by inducing the transcription of NANOG or NANOGP8. However, because only NANOGP8 was highly expressed in CRC cells and tumor tissues (Ishiguro et al., 2012; Jeter et al., 2011), we believe that the induced NANOG proteins in CRC cells by CM from ECs from different organs were encoded by NANOGP8. Of note, the OCT4‐luc construct contains the promoter regions of the OCT4 gene on chromosome 6, which is different from that of OCT4 pseudogenes (Suo et al., 2005). This evidence concerns the gene POU5F1 and colorectal carcinoma.