NANOGP8 and colorectal carcinoma: To determine the specific expression of NANOGP8 in CRC cells, we digested the RT‐PCR‐amplified cDNA fragments with AlwNI, a method described previously to distinguish mRNA transcripts of NANOG and NANOGP8 (Fig. S3) (Ishiguro et al., 2012; Zhang et al., 2013).