More importantly, we showed that the RT‐PCR products from LPEC CM‐treated CRC cells were all digested by AlwNI (Fig. 4C), suggesting that the increased mRNA levels we detected in Fig. 4B were transcribed by NANOGP8. Together, these data suggest that all CRC cell lines used in these studies expressed NANOGP8 with undetectable NANOG and that the treatment of LPEC CM had specifically increased NANOGP8 expression in CRC cells. The gene discussed is NANOG; the disease is colorectal carcinoma.