More importantly, analysis of a separate group of “high-risk” individuals (shown by the presence of at least two autoantibodies and dysglycemia or impaired glucose tolerance) revealed that higher levels of unmethylated INS DNA can be measured, even when compared to the progressors and nonprogressors, indicating the potential of circulating unmethylated INS DNA to stratify the risk of T1D. This evidence concerns the gene INS and Impaired glucose tolerance.