In contrast, two prostate cancer-associated SPOP mutants lacking the NLS sequence (SPOP-F125V-ΔNLS, SPOP-F133L-ΔNLS) accumulated exclusively in the cytoplasm as puncta pattern similar as SPOP-ΔNLS, but these mutants did not co-localize with GFP-INF2, possibly due to impaired interaction with INF2 (S3B Fig). Here, SPOP is linked to Familial prostate cancer.