Using MPtNPs as non-enzymatic labels for the secondary antibodies, a sandwich-type immunosensor array was constructed for carbohydrate antigen 125 (CA125), carbohydrate antigen 153 CA153) and carcinoembryonic antigen (CEA) tumor markers by covalent immobilization of capture antibodies onto a triple graphene modified-SPCE. The gene discussed is CEACAM5; the disease is neoplasm.