As shown in Fig. 2C, the CUL4B-Flag immunocomplex from hFOB1.19, U2OS or Saos-2 cells pulled down DCAF4 and DCAF11 (Fig. 2C), but not DCAF1, 8 and 15, indicating that these two DCAFs form complexes with CUL4 and DDB1 in osteosarcoma cells. The gene discussed is DCAF4; the disease is osteosarcoma.