DCAF11 and osteosarcoma: To identify the substrates of CRL4BDCAF11 E3 ligase in osteosarcoma cells, we constructed the pCDNA3-Flag-HA-DCAF11 vector, a two-epitope tagged vector designed to minimize unspecific binding during purification, which we transfected into hFOB1.19, U2OS and Saos-2 cells.