To this end, we examined the mRNA and protein levels of these seven Cullins in four human osteosarcoma cell lines, U2OS, MG63, Saos-2 and HOS, versus the osteoblast cell line hFOB1.19, a non-cancerous cell line that is commonly used as a control in osteosarcoma studies. This evidence concerns the gene CACUL1 and osteosarcoma.