Comparing the DCAF4 and DCAF11 protein levels in cell lysates and in the CUL4B-Flag immunocomplex revealed that DCAF11 was upregulated in the cell lysates of U2OS and Saos-2 compared with hFOB1.19 cells (Fig. 2C), and the same amount of CUL4B-Flag immunocomplex was able to pull down much more DCAF11 protein in osteosarcoma cells than in hFOB1.19 cells (Fig. 2C). This evidence concerns the gene DCAF11 and osteosarcoma.