In the present study, tenovin-1 (a wild-type p53 activator) pretreatment decreased NSCLC cell sensitivity to cisplatin and WP1130 co-treatment, indicating that the USP9X–p53 ubiquitination/degradation pathway is necessary for WP1130-mediated chemosensitization. This evidence concerns the gene TP53 and non-small cell lung carcinoma.