Subsequently, to investigate the aspirin associated apoptotic route via SHH/GLI1 inhibition at protein level, Western blot showed that Caspase-3, Bax and cleaved PARP increased in a dosedependent manner and that Bcl-2, an anti-apoptosis protein, decreased in the glioma cells treated by aspirin (Fig. 3B). This evidence concerns the gene BCL2 and central nervous system cancer.