Moreover, the proportion of CD44+CD54+ cells in tumor cells digested from xenograft was measured using cytometry assay, and showed that S87 OE xenograft tumor possessed less CD4+CD54+ cells compared with S87 Ctrl tumor, which was consistent with the findings in vitro, indicating the RNF43 OE remarkably suppresses CD44+CD54+ cancer stem-like cells (Fig. 4b). Here, RNF43 is linked to neoplasm.