We could detect no expression of paralogues in H460, with A2780 expressing PIWIL2 and relatively low levels of PIWIL4; however, the levels of neither were significantly altered by TEX19 depletion, suggesting that TEX19 regulation of PIWI gene expression may not be universal in all cancer cell types (Fig. 6b). The gene discussed is PIWIL1; the disease is cancer.