A targeted next-generation sequencing panel using molecular inversion probes (MIPs) was developed to identify high-penetrance mutations in the CRC predisposing genes APC, MLH1, MSH2, MSH6, PMS2, MUTYH and NTHL1. We applied this panel to a cohort of 140 early-onset Chinese CRC patients (diagnosed at or below the age of 35 years). Here, MSH2 is linked to colorectal carcinoma.