Our loss-of-function experiments indicated that down-regulation of ZEB1-AS1 not only could suppress cell proliferation and migration but also could induce cell apoptosis in bladder cancer cell lines 5637 and SW780 by using CCK-8, Edu assays, wound-healing assays, transwell assays, ELISA assays, Hoechst staining and flow cytometry assays. This evidence concerns the gene ZEB1 and urinary bladder cancer.