To address astrocyte endfeet as the interfering structure between the astroglial soma and the vasculature, the detection of collagen IV and STL-binding sites was combined with the immunolabeling of aquaporin 4 in the ischemia-affected striatum (Fig 9A–9A”‘) and neocortex (Fig 9B–9B”‘) originating from control animals. This evidence concerns the gene RNF217-AS1 and ischemia.