Because miR-5096 was specifically detected in glioma cells [10] and bioinformatics tools (BLASTN, https://blast.ncbi.nlm.nih.gov) identified a potential target site for miR-5096 on KCNJ10 gene (overlap, 91%), we examined the impact of this microRNA on Kir4.1 expression and functions in U87 cells, a human glioblastoma cell line derived from a human astrocytoma of grade IV [11], and the U251 glioblastoma (grade III-IV) model [12–15]. This evidence concerns the gene KCNJ10 and central nervous system cancer.