Given that there is a role for filopodia in invasion of cancer cells (i.e. invadopodia; [18, 28]), we examined the effect of Kir4.1 depletion, i.e. by miR-5096 loading, barium blockage or siRNA knockdown, on the invasion rate of the two cell lines by using Boyden chamber with matrigel-precoated filter inserts (Figure 5B). The gene discussed is KCNJ10; the disease is cancer.