Finally, these results were confirmed by independent experiments in which FAF1-overexpressing primary tumour cells derived from MMTV-PyMT transgenic mice (Fig. 5i) exhibited a significant decrease in lung metastasis without detectable changes in primary tumour growth following mammary fat pad injection in Friend Virus B (FVB) mice (Fig. 5j–m). Here, FAF1 is linked to neoplasm.