Given that the assessment of the endothelial phenotype in vivo in a reliable mouse model of endothelial dysfunction is essential for the preclinical profiling of vasoprotective compounds, the aim of the present study was to take advantage of the 3D MRI-based method to profile effects of MNA on functional phenotype of endothelium in ApoE/LDLR-/- mice in vivo, in comparison to standard vasoprotective treatment such as ACE-I (perindopril), that is known to display significant vasoprotective effect in various experimental models including atherosclerosis (Chłopicki and Gryglewski, 2005). Here, APOE is linked to atherosclerosis.