To give better information on CRC tumorigenesis and progression, instead of PCR and Western blotting methods in whole tumor pieces, for this study, we performed double immunolabeling for light and confocal microscopy in order to achieve a “cell by cell” analysis to obtain high quality subcellular localization data of AmotL2, FKBP51, and IQGAP1 proteins. This evidence concerns the gene AMOTL2 and neoplasm.