Compared with published gene expression profiles from CRC cell lines38, 39, gene set enrichment analysis (GSEA) revealed that, there existed a significant negative correlation between Wnt/β-catenin upregulated genes and downregulated genes following KDM3A/B depletion, highlighting the critical role of KDM3 in β-catenin/Tcf-mediated transcription (Fig. 1m). The gene discussed is KDM3A; the disease is colorectal carcinoma.