Consistent with current standards to document a new biomarker (Noel-Storr et al., 2014) we screened a second independent set of patient CSF samples by WB and ELISA and confirmed consistency of levels in controls and reduction of NPTX2 in AD (Figure 5E, Figure 5—figure supplement 4, and Figure 5—source data 2). This evidence concerns the gene NPTX2 and Alzheimer disease.