NES and neoplasm: Nestin protein expression by immunohistochemistry was consistently associated with higher histological grade (OR 2.7–14.3), larger tumours (OR 1.8–2.4, Series I-III), lymph node negativity (OR 3.3, Series III), and interval-detected compared with screening-detected tumours (OR 1.8–2.7, Series I-II) (Supplementary Table S3).