To define the precise tyrosine residues within LPP required for TGFβ-induced invasion of NMuMG-ErbB2 breast cancer cells, we constructed a panel of LPP mutants in which tyrosine (Y) residues at positions 245, 297/298 and 301/302 were mutated to phenylalanine residues (F), either independently or in combination (Fig. 9a). The gene discussed is TGFB1; the disease is breast cancer.