Indeed, in tumours of metastatic melanoma patients, CD8+ T-cells preferentially localised at the tumour periphery where there was high P-selectin, E-selectin and ICAM-1 expression and not at areas within the tumour lacking expression.32 This was also demonstrated in a murine melanoma model, where increased expression of adhesion molecules was associated with increased T-cell infiltration and tumour regression, and relapsed tumours exhibited loss of adhesion molecule expression which correlated with decreased T-cell infiltration.40 This evidence concerns the gene CD8A and metastatic melanoma.