To characterize the cell death induced by DHA in MM cells, we examined the occurrence of apoptosis by immunofluorescence, using the phosphatidylserine (PS)-binding annexin V (AV) and the vital dye propidium iodide (PI), in RPMI-8226 and OPM-2 cells cultured in the presence of increasing doses of DHA (50-200 μM) for 24 and 48 hours. Here, ANXA5 is linked to Miyoshi myopathy.