TARDBP and amyotrophic lateral sclerosis: Previously, as facilitated by our discovery that unlike the well-folded proteins following the “Salting-in” rule that protein solubility increases upon adding salts over the range of low salt concentrations (usually <300–500 mM), “insoluble” proteins could only be solubilized in aqueous solution with minimized salt concentrations21, 22, we have successfully studied the ALS-causing and aggregation-prone TDP-43 N-terminal and C-terminal prion-like domains23, 24.